neun primary antibody  (Millipore)

Journal: bioRxiv

Article Title: Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo

doi: 10.1101/2025.07.16.664392

Figure Lengend Snippet: a, Images showing the CA1 of the mouse hippocampus at 7-14 days after the injection of AAV encoding GEMINI. Scale bars: 50 μm. b, Comparison of GEMINI density across 7-14 days, where significant increase in GEMINI count was observed. c,d Comparison of the neuronal density (NeuN+ cells, c ) and immune response (GFAP immunofluorescence, d ) across 7-14 days after the injection of AAV encoding GEMINI. e,f, Images showing the CA1 ( left ), CA2/3 ( middle ), and DG ( right ) of the mouse hippocampus with ( e ) and without ( f ) intracellular GEMINI nucleation in neurons. The GEMINI+ group received intracranial injection of AAV encoding GEMINI, while the GEMINI– group received equivolume saline. GEMINI particles (JF 669 , red), neurons (anti-NeuN, green), astrocytes (anti-GFAP, magenta), and Nuclei (Hoechst, cyan), were stained and imaged. Scale bars: 100 μm. b-d, bars: mean; whiskers: s.d..

Article Snippet: The sections were then incubated with primary antibodies anti-NeuN (GeneTex, GTX132974) and anti-glial fibrillary acidic protein (anti-GFAP, Cell Signaling, #3670) overnight at 4°C with gentle shaking.

Techniques: Injection, Comparison, Immunofluorescence, Saline, Staining

Journal: bioRxiv

Article Title: Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo

doi: 10.1101/2025.07.16.664392

Figure Lengend Snippet: a, Images showing high-level GEMINI expression in the cortex (top) and hippocampus (bottom). Scale bars: 500/20 μm for low/high magnification. b,c Comparison of the neuronal density (NeuN+ cells) and immune response (GFAP immunofluorescence) between groups with and without GEMINI expression (n=5 mice per group). d, Traces of mice with and without GEMINI expression in an open-field arena. e-g, Comparison of total traveling distance ( e ), maximal speed ( f ), and average speed ( g ) between the GEMINI+ and – groups (n=6 mice per group). h, Traces of mice with and without GEMINI expression in a Y-maze. i, Comparison of time spent in the novel arm between the GEMINI+ and – groups (n=6 mice per group). b,c, Box bounds: 25th and 75th percentile; whiskers: minimum and maximum; squares: mean; and center lines: median. e-g, i, bars: mean; whiskers: s.d..

Article Snippet: The sections were then incubated with primary antibodies anti-NeuN (GeneTex, GTX132974) and anti-glial fibrillary acidic protein (anti-GFAP, Cell Signaling, #3670) overnight at 4°C with gentle shaking.

Techniques: Expressing, Comparison, Immunofluorescence

Journal: bioRxiv

Article Title: Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo

doi: 10.1101/2025.07.16.664392

Figure Lengend Snippet: a, Schematic of experimental design. AAV encoding GEMINI was unilaterally injected into the primary visual cortex (V1) of mice (GEMINI group). A positive control group received AAVs expressing diphtheria toxin subunit A (dtA) at a comparable dose (dtA group), while a negative control group was injected with equivolume saline (saline group). b,c, Schematic ( b ) and snapshot ( c ) of the horizontal ladder (HL) rung walking test. The test was conducted 14 days after the AAV injection. Scale bar: 2 cm. d,e, Comparison of ladder crossing time ( d ) and mean missed steps per trial ( e ) across the GEMINI, dtA and saline groups. f, Images of the brains post-fixation. GEMINI and saline groups have comparable size of both hemispheres, while the dtA group exhibited visible shrinkage on the dtA-injected ( left ) hemisphere. Scale bars: 5 mm. g, Images of the M1 regions from GEMINI, dtA, and saline. Neurons (anti-NeuN, green), Nuclei (DAPI, cyan), GEMINI particles (JF 669 , violet), and cells transduced by the dtA AAV (mCherry, red) were stained and imaged. The mice were sacrificed immediately after the HL tests. Scale bars: 50 μm. h, Comparison of the neuronal density (NeuN+ cells) among the GEMINI, dtA, and saline groups (3 mice per group). d,e,h, bars: mean; whiskers: s.d..

Article Snippet: The sections were then incubated with primary antibodies anti-NeuN (GeneTex, GTX132974) and anti-glial fibrillary acidic protein (anti-GFAP, Cell Signaling, #3670) overnight at 4°C with gentle shaking.

Techniques: Injection, Positive Control, Expressing, Negative Control, Saline, Comparison, Staining