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(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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Image Search Results


(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

Journal: bioRxiv

Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

doi: 10.64898/2026.01.22.698893

Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

Techniques:

(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

Journal: bioRxiv

Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

doi: 10.64898/2026.01.22.698893

Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

Techniques: Labeling

(a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

Journal: bioRxiv

Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

doi: 10.64898/2026.01.22.698893

Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

Techniques: Labeling